4OL4
Crystal structure of secreted proline rich antigen MTC28 (Rv0040c) from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-11-03 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 102.220, 102.220, 68.719 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.480 - 2.800 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.22280 |
Structure solution method | MIR |
RMSD bond length | 0.018 |
RMSD bond angle | 2.027 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.521 | 19.475 | 2.850 |
High resolution limit [Å] | 2.700 | 8.540 | 2.700 |
Rmerge | 0.016 | 0.530 | |
Rpim | 0.007 | 0.216 | |
Total number of observations | 2293 | 11478 | |
Number of reflections | 11663 | ||
<I/σ(I)> | 22.4 | 86.6 | 4.4 |
Completeness [%] | 99.7 | 91.7 | 100 |
Redundancy | 6.8 | 6.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.2M TMND, 0.1M Bis-tris Propane pH 9.0, 20% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |