4OKY
Crystal structure of PvuRts1I, a 5-hydroxymethylcytosine DNA restriction endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 160.210, 160.210, 45.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.710 - 2.900 |
| R-factor | 0.25373 |
| Rwork | 0.252 |
| R-free | 0.28695 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.487 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.710 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.120 |
| Number of reflections | 13311 |
| <I/σ(I)> | 12.9 |
| Completeness [%] | 98.1 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 286 | 1.128M NaH2PO4, 0.752M K2HPO4, 0.1M CAPS pH 10.5, 0.19M Li2SO4, 6%(v/v) glycerol , VAPOR DIFFUSION, SITTING DROP, temperature 286K |
