4OKU
Structure of Toxoplasma gondii proMIC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-06-29 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.434, 159.321, 41.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.390 - 3.200 |
R-factor | 0.2667 |
Rwork | 0.264 |
R-free | 0.31980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.656 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1565)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.400 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 8867 | |
Completeness [%] | 87.5 | 65.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.7 | 293 | 0.2 M (NH4)2SO4 and 20% PEG 3350, pH 3.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |