4OK9
Crystal structure of the single-stranded RNA binding protein HutP from Geobacillus thermodenitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-23 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 90.811, 90.811, 76.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.010 - 1.910 |
| R-factor | 0.17897 |
| Rwork | 0.176 |
| R-free | 0.23187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ZH0 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.071 | 0.449 |
| Number of reflections | 27972 | |
| <I/σ(I)> | 56 | 5.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 16 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 10%(W/V) PEG300, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
