4OK7

Structure of bacteriophage SPN1S endolysin from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	54.616, 120.597, 126.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
Rwork	0.208
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.107	0.064	0.587
Number of reflections	64722
<I/σ(I)>	7.8
Completeness [%]	98.1	97.7	97
Redundancy	6.1	5.7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	295	1.6 M ammonium sulfate and 0.1 M Na citrate, pH 4, VAPOR DIFFUSION, SITTING DROP, temperature 295K