4OK2
Crystal Structure of Alg17c Mutant Y258A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-02-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.954, 136.635, 88.856 |
| Unit cell angles | 90.00, 107.11, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.450 |
| R-factor | 0.19609 |
| Rwork | 0.193 |
| R-free | 0.25633 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.513 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 68133 | |
| Completeness [%] | 95.2 | 75.39 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1 M Tris, 5% 2-methyl-2,4-pentanediol (MPD), 10% Polyethylene glycol (PEG) 6000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
