4OJP

Crystal Structure of Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120 in Complex with Maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	123.439, 147.984, 171.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.700 - 1.948
R-factor	0.192
Rwork	0.192
R-free	0.22000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	SeMet Tailspike structure
RMSD bond length	0.008
RMSD bond angle	1.436
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.700	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.107	0.475
Number of reflections	224635
<I/σ(I)>	2	2
Completeness [%]	98.5	88
Redundancy	5.9	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		0.1 M Tris pH 7.0-7.6, 20% PEG-1000. Soaked with mother liquor containing 37% saturated maltose, VAPOR DIFFUSION, HANGING DROP