4OJN
Crystal structure of human muscle L-lactate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 147.140, 147.140, 333.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 2.400 |
| R-factor | 0.2149 |
| Rwork | 0.213 |
| R-free | 0.24954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 10.730 | 2.400 |
| Rmerge | 0.019 | 0.581 | |
| Number of reflections | 162753 | ||
| <I/σ(I)> | 22.8 | 85.5 | 2.9 |
| Completeness [%] | 99.5 | 82.4 | 97.2 |
| Redundancy | 7.2 | 6.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM Bis-Tris propane, pH 7.0, 20% PEG400, 100 mM lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
