4OIX
Crystal structure of truncated Acylphosphatase from S. sulfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-03-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0716 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.780, 39.510, 64.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.490 - 1.550 |
| R-factor | 0.1496 |
| Rwork | 0.147 |
| R-free | 0.17795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bje |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.058 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.780 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.044 | 0.061 |
| Number of reflections | 11780 | |
| <I/σ(I)> | 23.1 | 10.7 |
| Completeness [%] | 96.0 | 80.3 |
| Redundancy | 4.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG 8000, 8% ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
