4OIF
3D structure of Gan42B, a GH42 beta-galactosidase from G.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.840, 181.350, 196.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.859 - 2.448 |
| R-factor | 0.1365 |
| Rwork | 0.133 |
| R-free | 0.20450 |
| Structure solution method | using phases from it's GanBE323A mutant |
| Starting model (for MR) | 4OJW |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 2.580 |
| High resolution limit [Å] | 2.448 | 2.450 |
| Rmerge | 0.113 | 0.348 |
| Number of reflections | 91957 | |
| <I/σ(I)> | 11.7 | 4.3 |
| Completeness [%] | 96.3 | 88.6 |
| Redundancy | 5.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 16-18% PEG 3350, 250 mM NaCl and 100 mM Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292.0K |
