4OIE

West Nile Virus Non-structural Protein NS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-10
Detector	NOIR-1
Spacegroup name	P 31 2 1
Unit cell lengths	49.710, 49.710, 139.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.855 - 1.850
R-factor	0.2171
Rwork	0.215
R-free	0.26170
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.769
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	SHARP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.855	24.855	1.950
High resolution limit [Å]	1.850	5.850	1.850
Rmerge		0.056	0.548
Number of reflections	17797
<I/σ(I)>	10.5	10.7	1.3
Completeness [%]	99.7	97.8	100
Redundancy	5.6	6.1	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	20% PEG 2K MME, 0.1 M sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K