4OID
Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 |
| Unit cell lengths | 133.606, 133.606, 320.959 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.880 - 2.300 |
| R-factor | 0.15969 |
| Rwork | 0.157 |
| R-free | 0.20684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4njq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 94682 | |
| Completeness [%] | 86.0 | 98.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 295 | 30% PEG400, 0.1M Tris, 0.2M MgCl2, 0.1mM ZnCl2 , pH 8.0, Microbatch Crystallization, temperature 295K |
