4OIA

Crystal Structure of ICAM-5 D1-D4 ectodomain fragment, Space Group P4322

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9
Spacegroup name	P 43 2 2
Unit cell lengths	96.070, 96.070, 321.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.992 - 3.700
R-factor	0.2326
Rwork	0.230
R-free	0.27960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	3.700
Number of reflections	16867
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	294	100 mM Tris-HCl 8.5, 10% PEG4000 , VAPOR DIFFUSION, temperature 294K