4OHS

The structure of a far-red fluorescent protein, AQ143

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Spacegroup name	P 1
Unit cell lengths	50.967, 68.114, 132.788
Unit cell angles	98.38, 90.74, 110.47

Refinement procedure

Resolution	37.326 - 2.190
R-factor	0.1912
Rwork	0.190
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.987
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	131.100
High resolution limit [Å]	2.190
Number of reflections	72946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100mM Tris, 50mM lithium sulfate, 20% w/v PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K