4OGP
Structure of C-terminal domain from S. cerevisiae Pat1 decapping activator (Space group : P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2012-03-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.120, 88.460, 67.910 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 46.591 - 2.150 |
| R-factor | 0.2068 |
| Rwork | 0.204 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.991 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.140 |
| Number of reflections | 35407 | |
| Completeness [%] | 98.0 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 15% PEG6000, 7.5% MPD, 100 mM MES, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
