4OGG

Crystal Structure of Arabidopsis thaliana DJ-1d with glyoxylate as substrate analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Detector technology	CCD
Collection date	2011-12-19
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	83.685, 92.922, 138.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
Rwork	0.185
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pdv
RMSD bond length	0.005
RMSD bond angle	1.334
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.088	0.052	0.400
Number of reflections	139052
<I/σ(I)>	10.8
Completeness [%]	97.7	99.8	93.2
Redundancy	9.5	13	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6		0.1 M MES, 0.1 M sodium chloride, 16% PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP