4OFW
Crystal Structure of Arabidopsis thaliana DJ-1d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Detector technology | PIXEL |
| Collection date | 2011-10-31 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.556, 115.047, 115.531 |
| Unit cell angles | 90.00, 91.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.243 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pdv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.110 | 0.071 | 0.345 |
| Rmeas | 0.110 | ||
| Number of reflections | 117444 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 95.9 | 99.2 | 90.1 |
| Redundancy | 4.5 | 6.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 0.1 M sodium citrate, 15% 2-propanol, 16% PEG4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP |
