4OFK

Crystal Structure of SYG-2 D4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.16962
Spacegroup name	C 1 2 1
Unit cell lengths	61.812, 54.131, 63.623
Unit cell angles	90.00, 104.61, 90.00

Refinement procedure

Resolution	40.135 - 1.802
R-factor	0.1923
Rwork	0.191
R-free	0.21060
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.044
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	18855
<I/σ(I)>	24.3
Completeness [%]	100.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	295	1.3 M Ammonium sulfate, 0.1 M Tris pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K