4OFI
Crystal Structure of Duf (Kirre) D1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.330, 84.358, 82.633 |
| Unit cell angles | 90.00, 115.27, 90.00 |
Refinement procedure
| Resolution | 37.503 - 2.300 |
| R-factor | 0.1944 |
| Rwork | 0.192 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4of8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.716 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_740)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 41218 |
| <I/σ(I)> | 12.7 |
| Completeness [%] | 100.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 25% PEG 3,350, 0.2 M Sodium chloride, 0.1 M Bis-tris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
