4OFD
Crystal Structure of mouse Neph1 D1-D2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 118.962, 120.008, 119.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.895 - 3.940 |
R-factor | 0.2986 |
Rwork | 0.297 |
R-free | 0.32910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB Entries 4OF0 4of8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.860 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1456)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.940 |
Number of reflections | 7856 |
<I/σ(I)> | 18.9 |
Completeness [%] | 99.7 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 15% PEG 3,350, 3% Dextran sulfate, 0.1 M Sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |