4OFA
Structural basis for thymine glycosylase activity on T:O6-methylG mismatch by methyl-CpG binding domain protein 4: Implications for roles of Arg468 in mismatch recognition and catalysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.561, 55.534, 104.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.030 - 1.550 |
R-factor | 0.13694 |
Rwork | 0.134 |
R-free | 0.18212 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e9g |
RMSD bond length | 0.016 |
RMSD bond angle | 1.657 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.550 |
Number of reflections | 35660 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 33% ethylene glycol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |