4OF8
Crystal Structure of Rst D1-D2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.941, 77.935, 121.097 |
| Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
| Resolution | 44.570 - 1.902 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4of0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.966 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 77176 |
| <I/σ(I)> | 16.2 |
| Completeness [%] | 88.0 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 8% PEG 3,350, 0.1 M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
