4OF7

Crystal Structure of SYG-1 D1, Crystal Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	I 41 2 2
Unit cell lengths	129.179, 129.179, 141.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.302 - 2.100
R-factor	0.203
Rwork	0.202
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4of0
RMSD bond length	0.005
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1265))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	34491
<I/σ(I)>	25.6
Completeness [%]	98.0
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	32.5% PEG 3,350, 0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K