4OEY

Crystal structure of AR-LBD bound with co-regulator peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	150
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.525, 65.718, 70.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.420 - 1.830
R-factor	0.19622
Rwork	0.193
R-free	0.24921
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oea
RMSD bond length	0.020
RMSD bond angle	2.244
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.420
High resolution limit [Å]	1.830
Number of reflections	22611
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.6M magnesium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K