4OEJ

Structure of membrane binding protein pleurotolysin B from Pleurotus ostreatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9797
Spacegroup name	P 31 2 1
Unit cell lengths	71.580, 71.580, 174.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.560 - 2.200
R-factor	0.1908
Rwork	0.189
R-free	0.22130
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.370	87.370	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge	0.077	0.036	0.669
Number of reflections	27236
<I/σ(I)>	18.8	43.1	3.9
Completeness [%]	100.0	99.2	100
Redundancy	10.2	9.2	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M trisodium citrate, pH 5.6, 20% w/v PEG4000, 0.2 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M trisodium citrate, pH 5.6, 20% w/v PEG4000, 0.2 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K