4OE9

The crystal structure of the n-terminal domain of COMMD9

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979614, 0.976226
Spacegroup name	P 1
Unit cell lengths	28.490, 35.580, 54.300
Unit cell angles	104.03, 93.30, 91.11

Refinement procedure

Resolution	26.286 - 1.550
R-factor	0.1355
Rwork	0.134
R-free	0.16510
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	MOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	SOLVE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.740	1.580
High resolution limit [Å]	1.550	1.550
Rmerge		0.186
Number of reflections	27029
<I/σ(I)>		7.9
Completeness [%]	90.2	51.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	291.15	28% mPEG 5000, 0.2M citric acid, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K