4ODN
Structure of SlyD from Thermus thermophilus in complex with S2-plus peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.830, 40.550, 53.220 |
| Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
| Resolution | 28.412 - 1.598 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.19830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.412 | 1.640 |
| High resolution limit [Å] | 1.598 | 1.598 |
| Number of reflections | 22554 | |
| <I/σ(I)> | 12.74 | 2.29 |
| Completeness [%] | 99.3 | 97.7 |
| Redundancy | 6.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 20% PEG6000, 0.1 M citric acid, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
