4OD4
Apo structure of a UbiA homolog from Aeropyrum pernix K1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-10-22 |
| Detector | Pilatus detector |
| Wavelength(s) | 0.9790, 0.97920, 0.96380 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.090, 123.070, 423.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.783 - 3.301 |
| R-factor | 0.273 |
| Rwork | 0.272 |
| R-free | 0.30290 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.115 | 0.892 |
| Number of reflections | 55984 | |
| <I/σ(I)> | 14.1 | 2.1 |
| Completeness [%] | 99.4 | 98.5 |
| Redundancy | 6.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.1M Na2SO4, 0.1M sodium cacodylate pH 5.0, 26.5% (w/v) PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
