4OCN
Crystal Structure of the Rpn8-Rpn11 MPN domain heterodimer, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.042, 80.042, 386.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.250 |
R-factor | 0.2346 |
Rwork | 0.232 |
R-free | 0.28110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ocl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.178 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.276 | 48.827 | 2.370 |
High resolution limit [Å] | 2.249 | 7.110 | 2.249 |
Rmerge | 0.058 | 0.022 | 0.806 |
Total number of observations | 15273 | 69687 | |
Number of reflections | 61223 | ||
<I/σ(I)> | 20.3 | 23.6 | 1 |
Completeness [%] | 99.8 | 99.1 | 99.2 |
Redundancy | 7.8 | 6.9 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROPS | 6 | 291 | 50 mM MES, pH 6.0, 100 mM magnesium chloride, 21% PEG3350, VAPOR DIFFUSION, HANGING DROPS, temperature 291K |