4OCL
Crystal Structure of the Rpn8-Rpn11 MPN domain heterodimer, crystal form Ia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00753 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.258, 63.431, 100.111 |
| Unit cell angles | 100.08, 92.75, 90.62 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2X1P AND 2O95 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.733 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.440 | 48.978 | 2.520 |
| High resolution limit [Å] | 2.392 | 7.560 | 2.392 |
| Rmerge | 0.081 | 0.043 | 0.634 |
| Total number of observations | 4455 | 19201 | |
| Number of reflections | 41691 | ||
| <I/σ(I)> | 10.1 | 14.1 | 1.2 |
| Completeness [%] | 96.4 | 96.5 | 90.4 |
| Redundancy | 3.5 | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROPS | 6 | 291 | 50 mM MES, pH 6.0, 200 mM calcium acetate, 22% PEG3350, VAPOR DIFFUSION, SITTING DROPS, temperature 291K |
