4OCE
Crystal structure of the disulfide oxidoreductase DsbA from Proteus mirabilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 37.830, 37.830, 298.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.530 - 1.768 |
| R-factor | 0.1487 |
| Rwork | 0.148 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.640 | 1.860 |
| High resolution limit [Å] | 1.768 | 1.768 |
| Rmerge | 0.122 | 0.565 |
| Number of reflections | 22817 | |
| <I/σ(I)> | 16.1 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG3350, Sodium Malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
