4OBX
Crystal structure of yeast Coq5 in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97882 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.270, 75.640, 84.480 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 49.040 - 2.200 |
| R-factor | 0.20672 |
| Rwork | 0.205 |
| R-free | 0.24785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4obw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.068 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.040 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 61136 | |
| Completeness [%] | 98.7 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289.15 | 20% (w/v) 2-propanol, 20% (w/v) polyethylene glycol 4000, 0.1 M sodium citrate tribasic, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |
