4OBW
crystal structure of yeast Coq5 in the SAM bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.340, 74.960, 84.080 |
| Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
| Resolution | 42.690 - 2.400 |
| R-factor | 0.23622 |
| Rwork | 0.234 |
| R-free | 0.28271 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.904 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.690 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 43628 | |
| Completeness [%] | 92.7 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289.15 | 20% (w/v) polyethylene glycol 3350, 0.2 M ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |
