4OAL
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with phenylurea inhibitor CPPU in alternative spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979340 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.660, 112.620, 203.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.250 - 1.900 |
| R-factor | 0.2078 |
| Rwork | 0.207 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o95 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.300 | 27.300 | 2.010 |
| High resolution limit [Å] | 1.900 | 5.660 | 1.900 |
| Number of reflections | 101537 | ||
| <I/σ(I)> | 11.44 | 34.66 | 1.42 |
| Completeness [%] | 99.7 | 99.6 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0,1M HEPES pH 7,5, 50% MPD, cocrystallized with 3mM CPPU in DMSO and 5% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
