4O9A
Crystal structure of Beta-ketothiolase (PhaA) from Ralstonia eutropha H16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.271, 104.705, 106.907 |
| Unit cell angles | 90.00, 106.12, 90.00 |
Refinement procedure
| Resolution | 27.860 - 1.520 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dlu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.860 |
| High resolution limit [Å] | 1.520 |
| Number of reflections | 200802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 295 | 25% PEG 3350, HEPES, 0.2M Ammonium sulfate, pH 7.5, hanging drop, temperature 295K |
