4O93
Crystal structure of Thermus thermophilis transhydrogeanse domain II dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.408, 86.920, 98.978 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 98.670 - 2.770 |
| R-factor | 0.20713 |
| Rwork | 0.204 |
| R-free | 0.26858 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.772 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 98.700 |
| High resolution limit [Å] | 2.770 |
| Number of reflections | 24865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipidic subic phase | 8.5 | 298 | 100 mM Tris pH 8.5, 350 mM NH4-formate, 100mM Na-thiocynate, and 18% (v/v) 1-4-butanediol, lipidic subic phase, temperature 298K |
