4O8W
Crystal Structure of the GerD spore germination protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.748, 98.450, 127.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.470 - 2.293 |
| R-factor | 0.20283 |
| Rwork | 0.200 |
| R-free | 0.25594 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.470 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.089 | 0.463 |
| Number of reflections | 34360 | |
| <I/σ(I)> | 30.5 | 6.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.4 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris-HCl, 15% ethyl alcohol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
