4O8N

Crystal structure of SthAraf62A, a GH62 family alpha-L-arabinofuranosidase from Streptomyces thermoviolaceus, in the apoprotein form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-01-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.807, 65.579, 84.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.452 - 1.648
R-factor	0.1286
Rwork	0.127
R-free	0.16300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.333
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1538))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.680
High resolution limit [Å]	1.648	1.648
Number of reflections	39537
<I/σ(I)>	29.96	4
Completeness [%]	96.2	92.7
Redundancy	5.9	92.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	18 mg/mL SthAraf62A, 0.1 M Bis-tris propane pH 6.5, 20% PEG 5K MME, VAPOR DIFFUSION, SITTING DROP, temperature 298K