4O8B
Crystal structure of transcriptional regulator BswR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.965, 43.915, 35.019 |
| Unit cell angles | 90.00, 115.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lfp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.980 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 3818 | |
| <I/σ(I)> | 6.4 | 2.9 |
| Completeness [%] | 97.3 | 83.5 |
| Redundancy | 4.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 20% w/v Polyethylene glycol 10000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
