4O7O
Crystal structure of Mycobacterium tuberculosis maltose kinase MaK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 96.610, 96.610, 459.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.308 - 2.401 |
| R-factor | 0.2085 |
| Rwork | 0.207 |
| R-free | 0.24380 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.249 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.082 | 0.548 |
| Number of reflections | 51256 | |
| <I/σ(I)> | 35.3 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M Bis-Tris, 1M (NH4)2SO4, 1% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
