4O7K

Crystal structure of Oncogenic Suppression Activity Protein - A Plasmid Fertility Inhibition Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-08-31
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	60.869, 60.869, 126.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.537 - 1.748
R-factor	0.1986
Rwork	0.197
R-free	0.23570
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX (CDE)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.748	1.748
Rmerge	0.057	0.433
Number of reflections	24068
Completeness [%]	96.2	80.1
Redundancy	25.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.2	293	10% Isopropanol, 0.5M lithium sulphate, 0.1M phosphate-citrate buffer pH 4.2, 0.2M NDSB-201, VAPOR DIFFUSION, temperature 293K