4O6O
Structural and functional studies the characterization of Cys4 Zinc-finger motif in the recombination mediator protein RecR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Wavelength(s) | 0.9875 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.604, 67.912, 94.364 |
| Unit cell angles | 90.00, 92.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vdp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.946 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 21645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 8%(W/V) PEG3350, 200MM LITHIUM CITRIC TRIBASIC TETRAHYDRATE, 100MM MES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
