4O6N
Structure of AF2299, a CDP-alcohol phosphotransferase (CDP-bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2013-10-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.329, 90.670, 106.444 |
| Unit cell angles | 90.00, 92.07, 90.00 |
Refinement procedure
| Resolution | 46.299 - 2.100 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26170 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.620 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | PHENIX (dev_1558) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 2.175 |
| High resolution limit [Å] | 2.000 | 2.100 |
| Rmerge | 0.124 | 1.620 |
| Number of reflections | 50389 | |
| <I/σ(I)> | 7.43 | 0.81 |
| Completeness [%] | 98.1 | 98.08 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Lipidic cubic phase (LCP) | 7.5 | 298 | 25% (v/v) PEG400, 0.1M HEPES pH 7.5, 0.2M Na/K tartrate, Lipidic cubic phase (LCP), temperature 298K |
