4O6D
West Nile Virus Non-structural protein 1 (NS1) Form 1 crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-11 |
| Detector | MARMOSAIC 300 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 168.689, 168.689, 92.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.794 - 2.594 |
| R-factor | 0.1639 |
| Rwork | 0.163 |
| R-free | 0.19110 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.091 | 1.558 |
| Number of reflections | 47838 | |
| <I/σ(I)> | 21.1 | 1.2 |
| Completeness [%] | 99.9 | 94.8 |
| Redundancy | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 25% PEG 3000, 5% Glycerol, 20 mM Sodium Citrate pH 5.5, vapor diffusion, sitting drop, temperature 278K |
