4O66

Crystal Structure of SMARCAL1 HARP substrate recognition domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	193
Detector technology	CCD
Collection date	2011-10-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	56.511, 56.634, 104.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.772 - 1.900
R-factor	0.1713
Rwork	0.169
R-free	0.19660
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.306
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.093	0.464
Number of reflections	26465
<I/σ(I)>	26.2	4.4
Completeness [%]	97.0	96.4
Redundancy		9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294.2	0.2M ammonium sulfate, 0.1M MES, 30% PEG monomethyl ether 5,000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.2K