4O60
Structure of ankyrin repeat protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2013-10-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.463, 46.801, 74.249 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 38.310 - 2.520 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xee |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 27744 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 298 | 100 mM sodium acetate trihydrate (pH 4.5), 200 mM (NH3)2SO4, 16-18% PEG 4000, and 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
