4O4O

Crystal structure of phycobiliprotein lyase CpcT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-21
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 31 2 1
Unit cell lengths	69.322, 69.322, 165.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.533 - 1.950
R-factor	0.175
Rwork	0.173
R-free	0.21360
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Rmerge	0.065
Number of reflections	33803
<I/σ(I)>	58.7
Completeness [%]	97.9
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	MgCl2 6H2O (0.1M), Bis-Tris (0.1 M, pH 5.5) and polyethylene glycol 3350 (14% w/v), VAPOR DIFFUSION, HANGING DROP, temperature 293K