4O42
Tandem chromodomains of human CHD1 in complex with influenza NS1 C-terminal tail dimethylated at K229
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-03 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.068, 46.057, 46.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.060 - 1.870 |
| R-factor | 0.1905 |
| Rwork | 0.189 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b2w |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.060 | 46.060 | 1.910 |
| High resolution limit [Å] | 1.870 | 8.970 | 1.870 |
| Rmerge | 0.087 | 0.012 | 0.012 |
| Total number of observations | 1288 | 9282 | |
| Number of reflections | 20229 | ||
| <I/σ(I)> | 13.3 | 31.9 | 1.6 |
| Completeness [%] | 100.0 | 98.4 | 100 |
| Redundancy | 7.1 | 5.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | 15% PEG3350, 0.1 succinic acid, pH 7, temperature 291K |
