4O2X

Structure of a malarial protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	200
Detector technology	PIXEL
Collection date	2013-08-09
Detector	PSI PILATUS 6M
Spacegroup name	P 61
Unit cell lengths	97.850, 97.850, 298.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.670 - 2.700
R-factor	0.189
Rwork	0.185
R-free	0.23900
Structure solution method	SAD + MR
Starting model (for MR)	PDB ID 3W3I 1mg9 1MBX and 3DNJ
RMSD bond length	0.009
RMSD bond angle	1.232
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: dev_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	73.703
High resolution limit [Å]	2.699
Rmerge	0.040
Number of reflections	43001
<I/σ(I)>	15.8
Completeness [%]	97.4
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	273	Ammonium Sulfate 2 M, NaCl 1.8 M no buffer, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 273K