4O1I
Crystal Structure of the regulatory domain of MtbGlnR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 41 |
| Unit cell lengths | 144.241, 144.241, 92.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.860 - 2.800 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.429 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.098 | 0.052 | 0.319 |
| Number of reflections | 23093 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 99.0 | 97.2 | 99.7 |
| Redundancy | 3.1 | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 1.8M sodium acetate trihydrate, 0.1M Bis-Tris propane, pH 7.0, vapor diffusion, temperature 277K |
